ABSTRACT
Statement of the Problem: Sinonasal papilloma [SNP] is a rare benign lesion characterized by high recurrence rate and malignant transformation
Purpose: This study aimed to investigate the prevalence of human papilloma virus [HPV] infection in these lesions in South of Iran
Materials and Method: In this cross sectional retrospective study, a total of 41 patients, 38 SNP and 3 SNP/Squamous cell carcinoma cases, from 2007 to 2014 were studied. Human papilloma virus [HPV] DNA detection was performed by nested PCR method and positive cases were analyzed for high risk HPV-16 and HPV-18
Results: HPV was detected in 31.7%; HPV- 16 in 4.9% and HPV 18 was not detected at all. Dysplastic epithelium was detected in 53% that was not associated with HPV. Three cases were accompanied with malignant transformation that HPV genome was detected in only one case and none of them were positive for HPV16 /18 genomic DNA
Conclusion: Current research suggests that HPV may be involved in the development of SNP. But the high risk HPV is not important in malignant transformation. More studies are needed to elucidate the possible etiologic mechanism between HPV, inverted papilloma, and squamous cell carcinoma
ABSTRACT
Alterations in the expression of microRNAs [miRNAs] have been proposed to play a role in the pathogenesis of acute lymphoblastic leukemia [ALL] and chronic lymphocytic leukemia [CLL]. Dicer is one of the main regulators of miRNA biogenesis, and deregulation of its expression has been indicated as a possible cause of miRNA alterations observed in various cancers. Our aim was to analyze the expression of the Dicer protein and its relationship with ALL and CLL. This cross-sectional study was performed from 2010 to 2012 in Shahid Faghihi Hospital, Shiraz, Iran. In this study, 30 patients with CLL, 21 patients with ALL, 10 child healthy donors, and 19 adult healthy donors were recruited. The patients' samples were checked via flow cytometry, immunohistochemistry, and immunocytochemistry. The controls' samples were also examined in the hematology ward. Total RNA was extracted from the bone marrow and peripheral blood samples of the patients and controls. Then, reverse-transcription polymerase chain reaction was used to estimate the level of Dicer miRNA. The outcomes of the expression analysis of Dicer revealed statistically significant differences between the ALL patients/child healthy controls [meaniSD, 0.19 +/- 0.28vs. 0.73 +/- 0.12; P<0.001] and the CLL patients/adult healthy controls [mean +/- SD, 0.24 +/- 0.25 vs. 0.41 +/- 0.28; P=0.033]. This is the first piece of evidence showing that the expression of the Dicer gene greatly decreased in the patients with ALL in comparison to the child controls. The expression of the Dicer gene was also downregulated in the patients with CLL compared to the adult controls. Given the above findings, the expression of Dicer may play an important role in the progression and prognosis of these diseases